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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/03/2009 |
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Data da última atualização: |
08/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JOSÉ, A. A. F. B. V.; GAMA, M. A. S.; LANNA, D. P. D. |
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Afiliação: |
A. A. F. B. V. José, ESALQ/USP; Marco Antonio Sundfeld da Gama, Embrapa Gado de Leite; Dante Pazzanese Duarte Lanna, ESALQ/USP. |
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Título: |
Effects of trans - 10, cis - 12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 7, n. 2, p. 284-294, 2008. |
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DOI: |
https://doi.org/10.4238/vol7-2gmr417 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue. MenosThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12... Mostrar Tudo |
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Palavras-Chave: |
Adipose tissue explants; Bioactive fatty acid. |
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Thesaurus Nal: |
conjugated linoleic acid; lipogenesis; swine. |
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Categoria do assunto: |
-- |
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URL: |
https://www.funpecrp.com.br/gmr/year2008/vol7-2//pdf/gmr417.pdf
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Marc: |
LEADER 02768naa a2200217 a 4500
001 1596283
005 2024-02-08
008 2008 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/vol7-2gmr417$2DOI
100 1 $aJOSÉ, A. A. F. B. V.
245 $aEffects of trans - 10, cis - 12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs.$h[electronic resource]
260 $c2008
520 $aThe objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue.
650 $aconjugated linoleic acid
650 $alipogenesis
650 $aswine
653 $aAdipose tissue explants
653 $aBioactive fatty acid
700 1 $aGAMA, M. A. S.
700 1 $aLANNA, D. P. D.
773 $tGenetics and Molecular Research$gv. 7, n. 2, p. 284-294, 2008.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/01/2008 |
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Data da última atualização: |
17/01/2008 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Circulação/Nível: |
-- - -- |
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Autoria: |
XAVIER, J. H. V.; OLIVEIRA, M. N. de; ALMEIDA, S. C. R. de; GASTAL, M. L.; ROCHA, F. E. de C.; SILVA, F. A. M. da; SCOPEL, E. |
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Afiliação: |
José Humberto Valadares Xavier, CPAC; Marcelo Nascimento de Oliveira, CPAC; Suênia Cibeli Ramos de Almeida, CPAC; Marcelo Leite Gastal, CPAC; Francisco Eduardo de Castro Rocha, CPAC; Fernando Antônio Macena. |
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Título: |
Adaptação e utilização de dispositivo metodológico participativo visando o desenvolvimento sustentável de assentamentos de reforma agrária. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE SISTEMAS DE PRODUÇÃO, 7., 2007, Fortaleza. Agricultura familiar, políticas públicas e inclusão social. Fortaleza: Embrapa Agroindústria Tropical, 2007. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Desenvolvimento. |
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Thesagro: |
Pesquisa; Planejamento Participativo; Reforma Agrária. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00883naa a2200229 a 4500
001 1570342
005 2008-01-17
008 2007 bl uuuu u00u1 u #d
100 1 $aXAVIER, J. H. V.
245 $aAdaptação e utilização de dispositivo metodológico participativo visando o desenvolvimento sustentável de assentamentos de reforma agrária.
260 $c2007
650 $aPesquisa
650 $aPlanejamento Participativo
650 $aReforma Agrária
653 $aDesenvolvimento
700 1 $aOLIVEIRA, M. N. de
700 1 $aALMEIDA, S. C. R. de
700 1 $aGASTAL, M. L.
700 1 $aROCHA, F. E. de C.
700 1 $aSILVA, F. A. M. da
700 1 $aSCOPEL, E.
773 $tIn: CONGRESSO BRASILEIRO DE SISTEMAS DE PRODUÇÃO, 7., 2007, Fortaleza. Agricultura familiar, políticas públicas e inclusão social. Fortaleza: Embrapa Agroindústria Tropical, 2007. 1 CD-ROM.
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